Banca de QUALIFICAÇÃO: YASMIN FERNANDA NUNES VIANA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : YASMIN FERNANDA NUNES VIANA
DATA : 07/07/2023
HORA: 13:30
LOCAL: Bloco I, cidade universitária - UNEMAT, Cáceres, MT
TÍTULO:

DEVELOPMENT OF A PROTOCOL FOR RAPID IDENTIFICATION OF TEAK (Tectona grandis L. f.) Ceratocystis fimbriata USING RT-qPCR

PALAVRAS-CHAVES:

 Molecular diagnosis; Ceratocys wilt; Biotechnological development.

PÁGINAS: 30
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Melhoramento Vegetal
RESUMO:

Tectona grandis L. f is a forest species of high economic value, used in the manufacture of luxury furniture and shipbuilding. In Mato Grosso, this exotic species has adapted well to the climate, presenting a high productive potential. However, the teak crop has been threatened by Ceratocystis wilt, a fungal disease that can lead to loss of production. The objective of this project is to develop a quick and simple protocol for the identification of Ceratocystis fimbriata in teak wood using RT-qPCR. The work will be carried out between August 2022 and December 2023, in the laboratories of the Graduate Program in Genetics and Plant Improvement, in partnership with the company TRC – Teak Resources LTDA. For DNA extraction, simple and fast protocols will be used, with adaptations for the use of the DNA extraction kit Wizard® Genomic DNA Purification Kit (Promega). Confirmation of the quantity and quality of the extracted RNA will be performed by electrophoresis in a 1% agarose gel and will be compared with standard ʎ RNA (lambda), with a Qubit® 2.0 Fluorimeter (Invitrogen). The Reverse Transcription (RT) procedure will be used to generate the DNA that will be used in real-time PCR, which will allow the identification of the pathogen present in these samples. 20 primers and the fluorescent compound SYBR® Green will be used. It is expected to develop a simple and effective protocol for the rapid identification of Ceratocystis wilt in Teak, thus contributing to the genetic improvement program of this species.

MEMBROS DA BANCA:
Presidente - 285519001 - THIAGO ALEXANDRE SANTANA GILIO
Interno - 130513001 - SABRINA CASSARO
Externo ao Programa - 313571001 - MARIA DO SOCORRO BEZERRA DE ARAUJO