Banca de QUALIFICAÇÃO: GISEUDO APARECIDO DE PAIVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : GISEUDO APARECIDO DE PAIVA
DATA : 20/06/2022
HORA: 08:00
LOCAL: Unemat Alta Floresta
TÍTULO:

MOLECULAR CHARACTERIZATION OF LOCAL MANIOC VARIETIES CULTIVATED BY UARINI FLOUR PRODUCERS

 IN THE TEFÉ REGION,  AMAZONAS BRAZIL

PALAVRAS-CHAVES:

Genetical diversity; cassava; Uarini flour

PÁGINAS: 19
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:

Cassava (Manihot esculenta Crantz) belongs to the family Euphorbiaceae Juss., and to the genus Manihot Mill.,

 is a very widespread crop throughout Brazil, and plays an important role in generating employment and income.

 In this sense, this study aims to characterize the genetic diversity of cassava ethnovarieties maintained and 

managed by traditional farmers involved in the Uarini flour chain through SSR molecular markers. This research

 is part of the project: “Interdisciplinarity to strengthen the Uarini flour production chain - Ethnobotany and genetic

 diversity of Manihot esculenta Crantz”, approved in BID N. 003/2020 – PAINTER/FAPEAM, coordinated by 

Dr. Juliana Rodrigues Larrosa Oler. The research will be developed in the geographical delimitation of the area 

determined by the Geographic Identification (GI) Uarini, which comprises the municipalities of Uarini, Alvarães, 

Tefé and Maraã, located in the state of Amazonas. In this study, each municipality will be considered as a

 population. Two young and fully expanded leaves will be collected from 20 accessions distributed by the 5 most

 frequent local cassava varieties in the farmers' crops in each municipality, making a total of 80 individuals in 

the four populations. Molecular analyzes will be carried out at the Laboratory of Plant Genetics and Molecular 

Biology of the University Campus of Alta Floresta, UNEMAT. Total genomic DNA will be extracted from 

approximately 100 mg of leaf material following the CTAB method described by Doyle & Doyle (1987) with 

modifications for the species. The amplification reactions will be performed with 10 microsatellite markers 

labeled with 6-FAM fluorochrome (blue) and HEX (green). Polymerase Chain Reactions (PCR), quantification

 and genotyping will be performed. The analysis of genetic diversity among local varieties will be carried out

 in two stages: considering the total number of individuals belonging to a single population and, later, at the 

population level, considering the four sampled municipalities.

MEMBROS DA BANCA:
Presidente - 131995001 - CELICE ALEXANDRE SILVA