Banca de DEFESA: Jéssica Karina Guedes Cavalcante

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : Jéssica Karina Guedes Cavalcante
DATA : 29/02/2024
HORA: 08:00
LOCAL: SALA DO PPGASP
TÍTULO:

ENVIRONMENTAL RISK OF INSECTICIDES APPLIED IN THE STATE OF MATO GROSSO AND THE USE OF ENTOMOPATHOGENIC BACTERIA AS A STRATEGY FOR MANAGEMENT PEST STINK BUG IN SOYBEAN

PALAVRAS-CHAVES:

ERI, Brown stink bug, Colony Forming Units, Product association, Pseudomonas fluorescens+Pseudomonas chlororaphis.

PÁGINAS: 80
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitossanidade
ESPECIALIDADE: Entomologia Agrícola
RESUMO:

 

Euschistus heros (Hemiptera: Pentatomidae), commonly called brown stink bug, is considered a key pest of soybean crops, due to the damage caused to pods and grains. Currently, its management is based, almost exclusively, on the spraying of chemical insecticides. Consequently, concern emerges regarding the environmental risk of these pesticides, which can be estimated through certain tools such as the Environmental Risk Index (ERI). Furthermore, in recent years, the application of entomopathogenic microorganisms (bacteria, fungi and viruses) associated with pesticides is also evident, with the aim of minimizing re-entry into the crop. However, data on the compatibility of these associations are scarce, especially in the control of E. heros. Thus, the present work aimed to: estimate the ERI of the main insecticides used to control stink bug pests in soybean crops, in Mato Grosso, and evaluate the toxicity of the association of entomopathogenic bacteria and phytosanitary products registered for the aforementioned crop, in the management from E. heros. Data on the most commercialized active ingredients (a.i.s.) for the management of stink bug in soybeans in 2022 were collected from the Mato Grosso Agricultural Defense Institute (INDEA-MT). The physicochemical characteristics of the active ingredients were obtained from the Pesticide Properties Database (PPDB) at the University of Hertfordshire. The parameters used to calculate the ERI were: persistence, leaching, volatilization, toxicological profile and dose. In the in vitro compatibility test, 24 phytosanitary products associated with Pseudomonas fluorescens+Pseudomonas chlororaphis were evaluated, while in the spray test, seven sprays were simulated, based on applications by soybean farmers in the region. Compatibility was determined by counting colony forming units (CFUs) and measuring colony diameter (cm). The associations considered compatible were used in vivo bioassays with 3rd instar nymphs and adults (females) of E. heros. From the results obtained, it was found that in 2022, 11.612.956 kg of chemical insecticides for soybeans were sold, with acephate representing ≅33,18% of this volume (3.853.687 kg). Regarding ERI, the highest index was observed for the neonicotinoid imidacloprid (ERI=12), while the lowest value was found for the organophosphate acephate (ERI=5) and the sulfoxamine sulfoxaflor (ERI=5). In in vitro bioassays, of the 24 products evaluated, only Beauveria bassiana and indoxacarb were compatible for P. fluorescens+P. chlororaphis. Regarding the sprays, they were all incompatible, inhibiting the growth of the bacteria under study. In in vivo tests, the combination of P. fluorescens+P. chlororaphis + indoxacarb proved to be the most efficient in controlling 3rd instar nymphs, in addition to significantly reducing fertility and fecundity parameters in adults. Given these results, it is expected that the ERI estimate will provide support for the selection of i.as. with low toxicity, in order to minimize the negative impacts arising from undue spraying, in addition, it was evident in the bioassays that the recommendation of the association between P. fluorescens+P. chlororaphis and herbicides/fungicides/adjuvants, as these were highly toxic to microorganisms. Finally, it is concluded that the a.i. indoxacarb can be applied in mixture with bacteria of the genus Pseudomonas in the management of the brown soybean stink bug.

MEMBROS DA BANCA:
Presidente - 101366004 - MONICA JOSENE BARBOSA PEREIRA
Interno - 118802007 - HILTON MARCELO DE LIMA SOUZA
Externo ao Programa - 80797005 - CARLA GALBIATI
Externo à Instituição - JOSÉ EDUARDO MARCONDES DE ALMEIDA - IB