Banca de DEFESA: JOSÉ BISPO DOS SANTOS NETO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOSÉ BISPO DOS SANTOS NETO
DATA : 14/07/2022
HORA: 08:00
LOCAL: meet.google.com/fxy-koiz-ukv
TÍTULO:

FEASIBILITY OF IN VIVO PRODUCTION OF BACULOVIRUS SPODOPTERA IN MODULAR STRUCTURES

PALAVRAS-CHAVES:

Biological control, Baculoviridae, Onfarm

PÁGINAS: 54
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:

The complexity of rearing structures is one of the main problems for biofactories and laboratories, because some entomopathogenic microorganisms such as spodoptera virus frugiperda multiple nucleopolyherovirus (SfMNPV) have as specific host only Caterpillars S. frugiperda, hindering the mass production of this biological agent "in vivo". Thus, the objective was to evaluate the in vivo production method of SfMNPV in S. frugiperda within modular structures, based on the preparation of a practical structure that met an average property of cereal cultivation in the state of Mato Grosso. The objective was to establish the best structural configuration, as well as quantify operational expenses and the feasibility of costs and processes. For this, modular structures were developed in containers for the production of caterpillars. Each container is the size of 06 meters (20 feet), with eight shelves of 100 x 40cm with individualized lighting between the divisions, for the creation of S. frugiperda, which holds cages for moths and plastic containers with artificial diet for caterpillars or for the allocation of caterpillars contaminated with the virus. Aiming at maintaining temperature (25°C), humidity (60%) and photophase (12 h), an automated control system was installed in each container working indefinitely for the setting of S. frugiperda. It was possible to achieve the maximum production within the rearing unit, with operational work of four people with a workload of 40 hours per week, an accumulated of about 55,000 individualized caterpillars, which were subsequently sent to the contamination unit, where they were contaminated at ages of 7.10,11 and 12 days, evaluating the best productive index by weight and quantifying the need for caterpillars necessary for the formulation of a commercial dose. The research demonstrated the best productivity contaminating caterpillars of 10 days of age resulting in a price of R$ 8.85 per dose for application per hectare, providing about 120000 doses per year for application, within the modulated modular structure, taking into account doses according to baculovirus assets already used commercially and approved by the ministries of agriculture,  for the control of S. frugiperda.    

MEMBROS DA BANCA:
Presidente - 136121002 - ALESSANDRA REGINA BUTNARIU
Interno - 279342001 - DANIELLE STORCK TONON
Externo à Instituição - LUIZ PANHOCA - UFPR