Banca de DEFESA: ALEXANDRA OLIVEIRA RAMOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ALEXANDRA OLIVEIRA RAMOS
DATA : 12/12/2023
HORA: 09:00
LOCAL: meet.google.com/gfb-vnce-ikx
TÍTULO:

Antifungal activity of Mato Grosso propolis and its inclusion in the Brazilian classification. 

PALAVRAS-CHAVES:

Phenolic content; Flavonoids; Antimicrobial; Antioxidant

PÁGINAS: 103
GRANDE ÁREA: Outra
ÁREA: Ciências Ambientais
RESUMO:

Propolis is a natural product, produced by bees, in its chemical composition there are compounds such as phenolics and flavonoids, which explain the biological antimicrobial and antioxidant activities. The general objective of the dissertation was to analyze propolis from Mato Grosso in terms of physicochemical characterization, and antimicrobial and antioxidant activities for inclusion in the Brazilian classification. The dissertation was divided into three articles to respond to the general objective. Propolis samples were collected in the municipalities of Cáceres, Comodoro, Conquista d'Oeste, Reserva do Cabaçal, Colíder, and Nova Santa Helena. The antimicrobial activity of propolis ethanolic extracts was evaluated using the broth microdilution method, at concentrations from 8000 to 1.95 ug/mL, expressed by the minimum inhibitory concentration (MIC). The physical-chemical characterization analyzed wax % (m/m), mechanical mass % (m/m), moisture % (m/m), ash % (m/m), oxidation index (seconds), quantification of total phenolics % (m/m) and total flavonoids % (m/m) and color. Antioxidant activity was analyzed by DHPP, FRAP, and ON methods. Data analysis was descriptive, expressed as mean and standard deviation. The physical-chemical characterization result showed that four propolis samples were not suitable for human consumption and three samples did not show antimicrobial activity. The propolis extracts from Mato Grosso have red, green, brown, and black colors, in addition, they have total phenolic and flavonoid contents, especially the olive green propolis from Reserva do Cabaçal. Antifungal activity was identified in propolis 1 with MIC at a concentration of 1000 ug/mL on Candida albicans, which had Borreria densifora as isolated pollen, distinct from the other samples. The other seven propolis samples did not show antifungal activity. Seven propolis samples from Mato Grosso were classified into 5 groups of Brazilian propolis, due to the similarity and biological activities presented, thus expanding the geographic origin of Brazilian propolis. Depending on the apiary location, red and green propolis from Mato Grosso have antimicrobial and antioxidant activity, and therefore can be included in the Brazilian propolis classification.

MEMBROS DA BANCA:
Presidente - 80797005 - CARLA GALBIATI
Interno - 118188003 - ERNANDES SOBREIRA OLIVEIRA JUNIOR
Externo à Instituição - MARIA CRISTINA MARCUCCI RIBEIRO -