Banca de DEFESA: ANA CAMILLA IGNACIO DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANA CAMILLA IGNACIO DOS SANTOS
DATA : 09/05/2022
HORA: 09:00
LOCAL: ENSINO REMOTO EMERGENCIAL
TÍTULO:

IMPACTS CAUSED BY PATHOGENIC AGENTS OF DOMESTIC DOGS ON THE WILD SPECIES Cerdocyon thous (Linnaeus, 1766) (CARNIVORA, CANIDAE) IN THE SERRA DAS ARARAS ECOLOGICAL STATION AND ITS SURROUNDINGS

PALAVRAS-CHAVES:

Conservation; Conservation Unit; Cross contamination; Parasites

PÁGINAS: 74
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:

The species Cerdocyon thous (Linnaeus, 1766) is widespread over a large part of the South American territory, in Brazil its record occurs in large part, except in the Amazon. It is a common species in the Cerrado biome, as it adapts to anthropized and peri-urban environments. Anthropogenic areas bring the populations of wild animals closer to the domestic ones, allowing the transmission of parasites. The aim of the study was to evaluate the prevalence of intestinal parasites in the populations of domestic and wild canids, as well as the possibility of cross-contamination. For the capture of Cerdocyon thous, 10 folding Tomahawk traps were installed, measuring 115 x 55 x 60. As bait, pieces of chickens placed at the bottom of the traps were used. Biological material. In relation to domestic dogs, a questionnaire was applied to the owners of the animals regarding their life habits and the collection of biological material was carried out without the need for sedation. The samples were properly packaged and kept refrigerated until further analysis in the laboratory. The results obtained in the stool samples, according to Hoffman (1934) were positive for several parasites in domestic dogs with frequency of occurrence for Blastocystis sp. 60%, Trichuris vulpis 10%, Dipylidium caninum 10%, Hookworm 20%, Giardia lamblia 20% and Sarcocystis sp. 20%. For C. thous, the frequency of occurrence of mite eggs was 28.57%, Hookworm 42.85%, Blastocystis sp. 28.57%, Trichuris vulpis 14.28%. With the FAUST technique, parasites of domestic dogs were observed with frequency of occurrence for Blastocystis sp. of 30% and Sarcocystis sp. 10%. For C. thous with a frequency of occurrence of Hookworm 42.85%, Blastocystis sp. 57.14%, Trichuris vulpis 14, 28% and Capillaria sp. 14.28%. Blood samples from domestic and wild canids were subjected to Reverse Transcription followed by Polymerase Chain Reaction (RT-PCR) to investigate pathogens (Anaplasma sp., Babesia sp., Erlichia canis, Erlichia sp, Hepatozon sp., Leishmania sp. and Trypanosoma sp and CDV). The results showed that 50% of samples from domestic dogs were positive for Anaplasma sp., 60% for Babesia sp., 30% for Erlichia canis and 20% for Erlichia sp. However, they were negative for the antigens Hepatozon sp., Leishmania sp., Trypanosoma sp. and CDV. For C. thous all samples had negative reactions for all genotypes analyzed. Serology for distemper antibody detection (rapid test kit) in serum samples from domestic and wild dogs revealed that 80% of the samples were reactive for domestic dogs and none for C. thous. The frequency of occurrence of parasites in the feces and the presence of pathogens of infectious diseases in the blood of domestic dogs can characterize a threat to wild dogs present in the Conservation Unit that have direct contact between them, as they have free transit between the Conservation Unit. and the surrounding areas. Considering that the home range of C. thous can reach more than 7 km2, which overlaps 60% of the sampled domestic dogs, this way it can contaminate not only the C. thous object of this study, but also Chrysocyon brachyurus (maned wolf), Lycalopex vetulus (field fox) and Speothos venaticus (vinegar dog), as well as the feline species present in the area. For example, Blastocystis sp. was present in both species, which may demonstrate the contact between the animals. Preservation measures imply a work of sensitization of some residents who own domestic dogs, related to the vaccination and deworming of these animals. Thus, we emphasize that the combination of analysis techniques seeking to identify pathogens and parasites that affect diseases in domestic and wild canids, in addition to the mode of contagion, is essential to propose measures for the conservation of wild fauna.

MEMBROS DA BANCA:
Presidente - 61265003 - ANTONIO FRANCISCO MALHEIROS
Interno - 118188001 - ERNANDES SOBREIRA OLIVEIRA JUNIOR
Interno - 001.165.931-99 - WILKINSON LOPES LAZARO - UFRJ
Externo à Instituição - DANIEL MOURA DE AGUIAR - UFMT