Banca de QUALIFICAÇÃO: RITHIELY CONCEIÇÃO SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : RITHIELY CONCEIÇÃO SILVA
DATA : 15/12/2021
HORA: 08:00
LOCAL: https://meet.google.com/xux-deac-zwv
TÍTULO:

Selection of honey from Cáceres (Brazil) with indication of mechanism of action against Enterococcus faecalis

PALAVRAS-CHAVES:

Antibacterial activity; Outer membrane permeability; Staphylococcus epidermidis; Helicobacter pylori

PÁGINAS: 70
GRANDE ÁREA: Outra
ÁREA: Ciências Ambientais
RESUMO:

This study evaluated the antibacterial activity of honey and fractions from Apis mellifera and the indicative mechanism of action of membrane permeability in combination with antibiotics. The honeys evaluated from the municipalities the state of Mato Grosso such as Cáceres (honey 1 and 2), Barra do Garças (honey 3), Nossa Senhora do Livramento (honey 4) and Sinop (honey 5). These honeys have higher phenolic content, antioxidant and antibacterial activity compared to others in the state, studied by the Laboratory of the Center for the Study of Beekeeping (CETApis). The five fresh honeys and their fractions (hexane, ethyl acetate and n-butanol) were evaluated for inhibition of the growth of Enterococcus faecalis, Staphylococcus epidermidis and Helicobacter pylori bacteria. The antibacterial activity was verified by microdilution in broth method, with serial dilution from 1000 to 1.95 μg/mL. The indicative of membrane permeability was investigated by microdilution in broth method, combining honey or fractions in serial concentrations of 80 to 0.2 mg/mL with the antibiotic (clarithromycin/erythromycin) in a concentration of 20 to 0.04 μg/mL, such as checkboard. Honeys and their fractions had no antibacterial activity up to a concentration of 1000 ug/mL. Honey 1 and its N-butanol fraction had an effect on membrane permeability for gram positive bacteria E. faecalis. Honeys and fractions had no effect on the membrane permeability of S. epidermidis. It is concluded that honey 1 from Cáceres are promising to enhance the antibacterial activity of the antibiotic linked to membrane permeability and flavonoid action.

MEMBROS DA BANCA:
Interno - 61265003 - ANTONIO FRANCISCO MALHEIROS
Interno - 83200001 - AUREA REGINA ALVES IGNACIO
Interno - 80797005 - CARLA GALBIATI
Externo ao Programa - 96130006 - LARISSA MARIA SCALON LEMOS