Leaf anatomy and histochemistry of six species of the genus Xylopia (Annonaceae) occurring in southern Amazonia
Adaptation, Comparison, Mato Grosso, secondary metabolites, plasticity.
As it has one of the richest biological diversity on the planet, Brazil has a vast potential for research with vegetables that substantiate secondary metabolites, among which we can mention the species of the genus Xylopia L. Xylopia produces a variety of secondary metabolites and has been investigated as a source potential of compounds that exhibit a wide variety of biological properties. Secondary metabolites are not directly related to plant growth and development, but assume functional characteristics that allow them to respond to environmental stimuli; in the same way that possible changes in the anatomy of species can perform functions that suggest a certain adaptive degree. In this sense, this study had as main objective to investigate Xylopia species occurring in different areas in the southern Amazon of Mato Grosso, seeking to characterize and compare anatomically and histochemically six species of the genus, in addition, we investigate possible adaptive characteristics of Amazonian Xylopia in different communities vegetables from the region. To carry out the study, we used leaves of tree species of the genus Xylopia, which were subjected to the usual methodologies for anatomical and histochemical research. The research is organized in two chapters, the first deals with the description and anatomical and histochemical comparison of the species: Xylopia amazonica, X. aromatica, X.benthamii, X. frutescens, X. neglecta and X. sericea, where it was possible to verify that the species have shared anatomical characteristics, such as paracitic stomata, epidermal cells with straight walls, thin cuticle, bulky substomatic chambers and secretory channels. As well as the results referring to histochemical tests, which also revealed similarity between species, since of the ten tests performed, five reacted positively in all species. The compounds present in the species, perform ecological functions related to adaptations to the environment, such as reduced sweating, water storage, defense against herbivory and antifungal action. Despite the large number of shared characteristics, it was possible to separate them into groups, where the species X. frutescens and X. sericea are the most similar among the six. The results obtained are able to assist in taxonomic studies in the circumstance in which they suggest greater kinship between the species X. frutescens and X. sericea and X. amazonica and X. aromatica. The second chapter revealed that four anatomical attributes differ between the studied sites, namely, stomatal size, central rib bundle thickness, palisade parenchyma thickness and leaf wing thickness, thus, the mean annual precipitation and the seasonality index also influenced the variation of these tissues, except the thickness of the leaf wing. Although some attributes do not vary between sites and are not related to environmental variables, they showed a higher value for intraspecific variability, such as the thickness of the adaxial and abaxial epidermis, stomatal density and thickness of the lacunous parenchyma. This study shows that X. amazônica presents a difference in the anatomical attributes between the different sites and that these differences are closely linked to environmental factors, more especially the average annual precipitation.