Banca de DEFESA: YASMIN FERNANDA NUNES VIANA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : YASMIN FERNANDA NUNES VIANA
DATA : 26/02/2024
HORA: 08:00
LOCAL: Bloco I, cidade universitária - UNEMAT, Cáceres, MT
TÍTULO:

DEVELOPMENT OF A PROTOCOL FOR RAPID IDENTIFICATION OF TEAK (Tectona grandis L. f.) Ceratocystis fimbriata USING RT-qPCR

PALAVRAS-CHAVES:

 Molecular diagnosis; Ceratocys wilt; Biotechnological development.

PÁGINAS: 60
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Melhoramento Vegetal
RESUMO:

Tectona grandis L. f. is a high-value forest species used in the production of luxury furniture and shipbuilding. In Mato Grosso, this exotic species has adapted well to the climate, showing high production potential. However, the cultivation of teak has been threatened by Ceratocystis wilt, a fungal disease that can lead to production loss. The objective of this project was to develop a rapid and simple protocol for the identification of Ceratocystis fimbriata in teak wood using RT-qPCR. The work was conducted between August 2022 and December 2023 in the laboratories of the Graduate Program in Plant Genetics and Breeding, in partnership with the company TRC – Teak Resources Ltd. For DNA extraction, simple and rapid protocols were used, with adaptations for the use of the Wizard® Genomic DNA Purification Kit (Promega). The confirmation of the quantity and quality of the extracted DNA was performed using 1% agarose gel electrophoresis and compared with the Qubit® 4.0 Fluorometer (Invitrogen). Ten specific primers were used for the C. fimbriata fungus. All bioinformatics work was done using NCBI, GeneBank, Blast, and Primer3 for primer design. Quantitative PCR was carried out on the BioRad CFX 96 using SYBR® Green. Of the 10 designed primers, eight worked adequately with the ability to identify the presence of DNA from the phytopathogenic fungus C. fimbriata at concentrations of 1 picogram. Thus, we conclude that the primers and protocol developed in this work can be used for accurate diagnosis of C. fimbriata wilt in teak."

MEMBROS DA BANCA:
Presidente - 285519001 - THIAGO ALEXANDRE SANTANA GILIO
Interno - 130513001 - SABRINA CASSARO
Externo à Instituição - ALEXANDER CLARO GARCIA - UFMT
Externo à Instituição - MARIA DO SOCORRO BEZERRA DE ARAUJO -