Banca de DEFESA: RENAN COLAVITE DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : RENAN COLAVITE DOS SANTOS
DATA : 14/03/2023
HORA: 07:30
LOCAL: Campus Alta Floresta, Sala 6 unidade 2
TÍTULO:

NFLUENCE OF ESSENTIAL OILS ON IN VITRO MICROPROPAGATION AND CYTOGENETIC ANALYSIS OF Catasetum sp.

PALAVRAS-CHAVES:

in vitro propagation; Catasetum sp.; essencial oils;

PÁGINAS: 50
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Melhoramento Vegetal
RESUMO:

In vitro propagation has been used in Brazil for just over 25 years, mainly to increase the production of seedlings 

with quality and uniformity. Essential oils are aromatic products of secondary metabolism of plants, extracted 

from seeds, barks or leaves, they present volatile substances, they have numerous utilities such as in food, 

cosmetic and sanitizing formulations, currently they are used in many works of plant tissue culture, with antifungal

 and antibacterial action, so this work aims to evaluate the influence of different doses of three essential oils

 (Clove, Melaleuca and Ginger) on the vegetative development of Catasetum sp., evaluate the material grown 

in vitro through cytogenetics , characterizing the genetic parameters and genetic diversity of the species. 

The experiment will be conducted at the Laboratory of Cytogenetics and Plant Tissue Culture at the State University

 of Mato Grosso Carlos Alberto Reyes Maldonado – Campus de Alta Floresta – MT. Germination tests will be

 carried out with the tetrazolium dye. Catasetum protocorms will be used, obtained from in vitro sowing in an 

alternative culture medium consisting of 30g L-1 of sucrose, 4g L-1 of activated charcoal, 

2g L-1 of B&G® fertilizer, 100 mL L-1 of coconut water (RODRIGUES, 2012). The culture medium will be

prepared in the laboratory and autoclaved at 121°C for 20 minutes, with adjusted pH, and then sterilized 

with the addition of sterilized essential oils (Melaleuca Oil, Clove Oil and Ginger Oil). The design used will be 

a completely randomized design, using protocorms of Catasetum sp., with 6 treatments 

(0, 1, 2, 3, 4 and 5 mL L-1 of tea tree oil, cloves and ginger), with 5 replications analyzing 20 seedlings 

per treatment.

MEMBROS DA BANCA:
Presidente - 129897002 - ISANE VERA KARSBURG
Externo ao Programa - 257913001 - ADRIANA MATHEUS DA COSTA DE FIGUEIREDO
Externo ao Programa - 238586018 - DARLEY APARECIDO TAVARES FERREIRA
Externo à Instituição - THAIS ROSELI CORRÊA -