Banca de DEFESA: ANA PAULA ROVEDA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANA PAULA ROVEDA
DATA : 20/02/2023
HORA: 13:00
LOCAL: Alta Floresta, MT.
TÍTULO:

Cytogenotoxicity, genetic diversity and population structure of Vochysia divergens Pohl (Vochysiaceae) in the Pantanal of Mato Grosso state, Brazil.

PALAVRAS-CHAVES:

Cambará; Medicinal plants; Microsatellites.

PÁGINAS: 84
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:

Vochysia divergens Pohl., popularly known as cambará, belongs to the Vochysiaceae family, is a tree species, naturally found in floodplains and riparian forests in the Pantanal. Its wood is used to make canoes, troughs and toys, and the species also has medicinal, ornamental and reforestation uses. Despite being widely used, there are no studies regarding its cytogenotoxic potential and genetic diversity. Thus, the present study aimed to evaluate the cytogenotoxic potential of aqueous extracts (infusion and decoction) of the stem bark of V. divergens, using the Allium cepa test, as well as to characterize the diversity and genetic structure of native populations in the Pantanal of Mato Grosso state, Brazil using microsatellite molecular markers. The evaluation of the cytogenotoxic potential was carried out with two types of aqueous extracts (infusion and decoction), with five different concentrations and two controls, being distilled water (negative control) and copper sulphate pentahydrate (positive control). The two aqueous extracts have cytogenotoxic potential on the cells of the apical meristem of Allium cepa. The exception was the concentration of 200 mg ml^-1 of the infused extract, since it did not differ statistically from the negative control, being indicated for human consumption. The genetic diversity and structure were evaluated in 152 individuals distributed in six natural populations in the Pantanal of Mato Grosso state, using seven microsatellite molecular markers. 33 alleles were amplified, with an average of 4.71 alleles per locus. The six populations present He lower than Ho. The fixation index was negative, which indicated greater heterozygosity in the populations. The PIC ranged from 0.110 to 0.729 and only one locus was uninformative. We found 4 rare and 13 exclusive alleles in the populations. The largest component of variation was within populations (52.78%) and the gene flow estimate was 0.26, indicating low gene flow among populations. The clusters generated three groups. It is observed that the genetic structure is not related to the geographical distance among populations. Vochysia divergens has cytogenotoxic potential and its native populations in the Pantanal of Mato Grosso state present levels of important genetic diversity to help in practices that ensure the management and conservation of the species.

MEMBROS DA BANCA:
Presidente - 523.249.231-49 - ANA APARECIDA BANDINI ROSSI -
Externo ao Programa - 203668004 - KELLI EVELIN MULLER ZORTEA
Externo ao Programa - 253925001 - SERGIO ALESSANDRO MACHADO SOUZA
Externo à Instituição - MAGALI GONCALVES GARCIA - UFPA