Banca de DEFESA: MELCA JULIANA PEIXOTO RONDON

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MELCA JULIANA PEIXOTO RONDON
DATA : 28/01/2022
HORA: 08:00
LOCAL: ambiente virtual Google meet
TÍTULO:

On-farm conservation of cassava, cuiabá, Mato Grosso: ethnobotanical aspects and genetic diversity

PALAVRAS-CHAVES:

traditional community, genetic erosion, plant genetic resources

PÁGINAS: 47
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Melhoramento Vegetal
RESUMO:

This research aims to analyze the on-farm conservation of cassava practiced by a community in the Baixada Cuiabana region, state of Mato Grosso, under the ethnobotanical and molecular aspects, through the study of the genetic diversity of the collection they maintain. Root and tuber cultures originating in tropical regions play an important role in Brazilian small-scale agriculture, especially cassava. In the Baixada Cuiabana region, there is a strong characteristic that traditional farmers have in common, which is their dedication to the production of cassava, with in-depth knowledge about the management of ethnovariety, production and processing. Farmers were interviewed about ethnobotanical aspects of cassava, applying a questionnaire with open questions regarding the characteristics of each ethnovariety maintained by the farmer, morphological and physiological aspects, and how the vine was acquired. The collected sheets for molecular characterizations with fluorescent microsatellites. The leaf tissues of the cassava species they maintained were inserted into 2.0 mL polypropylene tubes with loading buffer (containing 1 mL of saturated NaCL-CTAB solution, 70g of NaCL, 3g of CTAB dissolved in 200 mL of water distilled). Then, the material was identified and sent to the Phytochemical Laboratory of Embrapa Agrossilvipastoril, Sinop-MT, and stored in a freezer at -4ºC until DNA extraction. To analyze the genetic diversity of cassava ethnovarities, allele frequencies, number of alleles per locus (A), expected (He) and observed (Ho) heterozygosity, allele fixation index (f) and percentage of polymorphic loci were estimated. (%P) through the GDA program - Genetic Data Analysis. The mean for expected (He) and observed (Ho) heterozygosity stands out with significant values, 0.67 and 0.72, respectively, with observed heterozygosity was higher than expected in nine of the 15 loci analyzed. Farmers' ethnobotanical practices contributed to the high genetic diversity found in the collection. There are rare alleles within the evaluated population, which reinforces the importance of maintaining and expanding the on farm/in situ conservation of these collections in order to stop genetic erosion. The matrinxã ethnovarity stood out for being the most divergent among the collection.

MEMBROS DA BANCA:
Presidente - 607.835.671-20 - EULÁLIA SOLER SOBREIRA HOOGERHEIDE - USP
Interno - 32143004 - ANA APARECIDA BANDINI ROSSI
Interno - 055.314.176-75 - JOSE ANGELO NOGUEIRA DE MENEZES JUNIOR - UFV
Externo à Instituição - AUANA VICENTE TIAGO - UNEMAT
Externo à Instituição - JOYCE MENDES ANDRADE PINTO - EMBRAPA