Banca de DEFESA: MARCELO DIAS MACHADO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARCELO DIAS MACHADO
DATA : 05/03/2020
HORA: 08:00
LOCAL: Câmpus Universitário de Tangará da Serra
TÍTULO:

In vitro regeneration, morpho-agronomic and enzymatic traits of Passiflora cincinnata Mast triploid plants. 

PALAVRAS-CHAVES:
PÁGINAS: 106
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Melhoramento Vegetal
RESUMO:

The general objective of the work was the in vitro production from somatic embryogenesis using the endosperm, morpho-agronomic characterization and evaluation of the behavior of triploid plants in the production of resistance enzymes in Passiflora cincinnata. For the production of triploid plants via somatic embryogenesis, mature endosperm from the seed of P. cincinnata is used. The highest average of somatic embryo production was observed in the supplementation treatment with 18.1 μM of 2.4-D + 4.5 μM of BA, with an average number of nine embryos per explant at 30 days of culture. Seedlings regenerated in vitro for 45 days were grown at home and a confirmed ploidy level with a DNA amount of 4.38 pg and chromosome number 3n = 3x = 27 and the respective diploids (2n = 2x = 18). The flowering of the triploid plants occurred at 210 of cultivation and showed high variability in phenotypic expression due to the genetic variability provided by the increase in ploidy. Diploid and triploid plants described polymorphism (77%) in the morpho-agronomic descriptors. As the triploid plants exhibit heteromorphic leaves, predominantly trilobed with larger size and darker tonal width. The flowers were bigger, darker and more intense, with a predominance of purple color. The diversity of flowers found in triple plants, seven changes, suggests that the addition of ploidy causes changes in the expression of genes and causes the occurrence of phenotypically variable colorings of flowers, improving the ornate patterns of the species. The internal and external diameter of the crown cavity increased in the triploid plants. The fruits of the triploids had smaller width and number of seeds/fruit and greater length and thickness of the peel compared to diploid. Pollen analysis data show that diploid and triploid species have high rates of pollen viability, 98.21 and 97.82%, respectively. Physiological changes in the production of oxidative (peroxidase and polyphenol oxidase) and hydrolytic (β-1,3-glucanase) enzymes were observed in the triploid plants of P. cincinnata. The increase in ploidy affected the activity of enzymes used in plant resistance. As plant growth-promoting rhizobacteria, A. brasilense and B. subtilis, were used in the activation of enzymes related to the resistance and plant defense mechanisms in diploid and triploid plants. A. brasilense provided the highest values of activity induction of the enzymes peroxidase and polyphenol oxity and B. subtilis induced greater activity of β-1,3-glucanases in P. cincinnata. The ability to respond to the defense mechanisms of triploid plants produced in vitro with the use of P. cincinnata endosperm and the induction of biostimulants in the production of resistance enzymes, opens new approaches in the genetic improvement for the genus.

MEMBROS DA BANCA:
Externo à Instituição - DIEGO ISMAEL ROCHA - UFJ
Externo ao Programa - 131840001 - ILIO FEALHO DE CARVALHO
Presidente - 59687004 - MAURECILNE LEMES DA SILVA