Banca de DEFESA: THATIELEN FURINI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : THATIELEN FURINI
DATA : 01/03/2021
HORA: 14:00
LOCAL: Alta Floresta - Via Google Meet
TÍTULO:

Morphology and biometry of fruits and seeds and quantification of the nuclear DNA of Bixa arborea and Bixa orellana. 

PALAVRAS-CHAVES:

Amazon; Chromosomes; Annatto

PÁGINAS: 72
GRANDE ÁREA: Ciências Biológicas
ÁREA: Genética
SUBÁREA: Genética Vegetal
RESUMO:

Brazil is the country richest in biodiversity, changing the greatest wealth in plant species. Bixa arborea and Bixa orellana, are two similar plant species, of the Bixaceae family, with wide distribution in South America, and widely used by the traditional population. The species of the genus Bixa have a pigment in the seeds, which has been used by the native populations of America for a long time, mainly in the ornamentation of the body and objects. Today it is known that this pigment and the leaves of Bixa arborea and Bixa orellana are rich in carotenoids, antioxidant compounds and vitamins. Mandatory to the high concentration of phytochemicals, Bixa orellana is widely used in the food and textile industry (as a dye) and in the pharmaceutical and cosmetic industry, in addition to being used as herbal medicines by the population. Bixa arborea besides phytotherapic use, is also widely used in forest restoration and as a source of wood. In this context, it is extremely important to differentiate genetically and morphologically B. arborea from B. orellana, through the taxonomic description of the species and the quantification of nuclear DNA of the species, further expanding the knowledge about these species. It also sought to analyze whether there was a morphological and genetic difference between B. arborea specimens, to find out if there were hybrids in the analyzed population. For this, the biometrics of the fruits and the morphological description of the two species were carried out at the Laboratory of Cytogenetics and Tissue Culture and at the Herbarium of the Southern Amazon of the University of the State of Mato Grosso, Alta Floresta campus, in addition to analysis of the DNA content through flow cytometry. From this, it was possible to perceive that the two species present many anatomical similarities, basically differing one another by the aspect of the fruit: B. arborea presented papulous fruits or partially covered by non-flexible spines with length varying from 0.1 to 1.8 mm, while B. orellana presents fruits completely covered by flexible spines and with length varying from 4 to 9.6 mm. The average amount of seeds in the fruits of B. orellana was higher than that found in the fruits of B. arborea. The specimens of B. arborea columns point morphological differences, such as greater number of flower stamens (phenotype 3), presence of thorns in the fruit (phenotype 5 and 6), absence of thorns in the fruit (1 and 2) and presence of fruit with long mucronatiform apex, being the only phenotype with this characteristic. The amount of nuclear DNA showed a difference between the two species and neither between those belonging to B. arborea, however this difference was not summarized. This shows that the species are very close genetically and, therefore, very related. Thus, we can conclude that the two species have difference mean the size of the genome, with the shape of the fruit and the quantity of seeds being the most suitable to separate the two species. 

MEMBROS DA BANCA:
Presidente - 129897002 - ISANE VERA KARSBURG
Externo ao Programa - 238586012 - DARLEY APARECIDO TAVARES FERREIRA
Externo ao Programa - 86029011 - JOSE MARTINS FERNANDES
Externo à Instituição - RICARDO GALLO - UFRPE