Banca de DEFESA: JEFERSON GONÇALVES DE JESUS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JEFERSON GONÇALVES DE JESUS
DATA : 27/01/2021
HORA: 09:00
LOCAL: Cáceres - Mt
TÍTULO:

Genetic variability of Ceratocystis fimbriata isolates

PALAVRAS-CHAVES:

Genetic diversity, phylogeny, microsatellite markers

PÁGINAS: 51
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:

In the forestry sector, the attack by pests and diseases can occur mainly via irrigation, cultural treatments, lack of phytosanitary care with tools, contaminated propagating material, and others, among the diseases that occur most frequently in teak, it is worth mentioning that caused by gender Ceratocystis that causes loss in wood productivity and quality. For the prevention or management of diseases in plants, studies on the genetic variability of the population of the causal agent become extremely important, so the objective of this work was to investigate the genetic diversity of Ceratocystis fimbriata isolates from teak, using microsatellite markers. 70 isolates of the fungus were obtained from collections in the states of MT and PA. The fungus was grown in MYEA medium for about 14 days for subsequent DNA extraction, it was performed using the Wizard® Genomic DNA Purification Kit (Promega), products from this extraction were submitted to the PCR step, where for each sample used approximately 25 ng total DNA (5 μL), 2.5 μL of 10x PCR buffer, 0.75 μL of 50 mM MgCl2, 0.31 μL of 20 mM dNTP, 1.25U of taq polymerase, 2.5 μL of each primer and 13.68 μL H2O ultrapure qsp After PCR, 2.5% agarose gel electrophoresis was performed to produce the gel images for 14 microsatellite loci. The images of the gels with the DNA fragments, amplified and separated, were subjected to the verification of molecular weights to obtain data on the alleles, and with these and the parameters of genetic diversity and population structure were elaborated. It was possible to separate the isolates into four distinct groups, the last containing more than 50% of the isolates.

MEMBROS DA BANCA:
Interno - 285519001 - THIAGO ALEXANDRE SANTANA GILIO
Interno - 101376004 - MARCO ANTONIO APARECIDO BARELLI
Externo à Instituição - SANDRA DA COSTA PREISIGKE - UENF